Complete sequence of three alpha-tubulin cDNAs in Chinese hamster ovary cells: each encodes a distinct alpha-tubulin isoprotein.
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چکیده
منابع مشابه
Taxol-dependent mutants of Chinese hamster ovary cells with alterations in alpha- and beta-tubulin
Chinese hamster ovary cell mutants resistant to the microtubule stabilizing drug taxol were isolated in a single step. Of these 139 drug-resistant mutants, 59 exhibit an absolute requirement for taxol for normal growth and division, 13 have a partial requirement, and 69 grow normally without the drug. Two-dimensional gel analysis of whole cell proteins reveal "extra" spots representing altered ...
متن کاملMutations in alpha- and beta-tubulin affect spindle formation in Chinese hamster ovary cells
Two Chinese hamster ovary cell lines with mutated beta-tubulins (Grs-2 and Cmd-4) and one that has a mutation in alpha-tubulin (Tax-1) are temperature sensitive for growth at 40.5 degrees C. To determine the functional defect in these mutant cells at the nonpermissive temperature, they were characterized with respect to cell cycle parameters and microtubule organization and function after relat...
متن کاملTaxol-dependent Mutants of Chinese Hamster Ovary Cells with Alterations in a- and/ -Tubulin
Chinese hamster ovary cell mutants resistant to the microtubule stabilizing drug taxol were isolated in a single step. Of these 139 drug-resistant mutants, 59 exhibit an absolute requirement for taxol for normal growth and division, 13 have a partial requirement, and 69 grow normally without the drug. Twodimensional gel analysis of whole cell proteins reveal "extra" spots representing altered t...
متن کاملSequence and expression of Paracentrotus lividus alpha tubulin gene.
In an effort to study the regulation of tubulin gene transcription during sea urchin embryogenesis we isolated several genomic and cDNA clones encoding for a and j3 polypeptides (1,2). Here we report the sequence of a full length a tubulin cDNA. Comparison of the predicted amino acid sequence of the cDNA clone (a 1 tubulin), shown in Fig. 1, with that of other clones encoding for a different a ...
متن کاملMicrotubules containing acetylated alpha-tubulin in mammalian cells in culture
The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy after double-staining by 6-11B-1 and B-5-1-2, a monoclonal antibody specific for all alph...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1986
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.6.3.906